FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199492
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317450.6105404143327848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160700.3090686551686203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146570.2818929233855923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144550.061.9105721
GGTATCA63550.060.953761
TCAACGC180050.050.826474
ATCAACG180400.050.6958543
CAACGCA182350.050.0874985
AACGCAG186550.048.989366
TATCAAC197500.046.396872
CGCAGAG207100.043.9546288
ACGCAGA208200.043.7518237
GCAGAGT242300.037.6182569
AGAGTAC236150.030.72466910-11
CAGAGTA255400.025.6367210-11
AGTACTT242650.025.45132412-13
GAGTACT220600.024.89370212-13
ACGGGAC5400.024.241753
AGTACGG45400.023.91664912-13
GAGTACG44350.023.67796512-13
GTCTCGC6100.020.484461
GTACGGG53750.019.9264131
GTACTTT282900.019.59058414-15
Produced by FastQC (version 0.11.5)