FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199492
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA34550.043.2014275
AACGCAG36000.041.4597746
TCAACGC36500.040.5675624
CGCAGAG36350.040.5651178
ACGCAGA37500.039.48317
ATCAACG39100.038.020233
GTATCAA51200.031.9381731
GAGTACG43200.026.2903861
AGTACGG44100.025.7543432
ACGGGAC4200.024.0686193
TATCAAC64800.023.400272
CTGTCGC7800.022.8671079
GCAGAGT66600.022.496299
CGATACC9950.022.43199118-119
GTACGGG54100.022.3123911
TCTGTCG8300.021.4906068
AGAGTAC58300.020.59891910-11
TACGGGA22850.020.038272
CTCGCTC7750.019.181823
TACGGGG26250.019.028552
Produced by FastQC (version 0.11.5)