FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004898047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774830
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT145640.21497218380387406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107240.15829179477566227No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG100170.1478561085665618No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT98770.14578963604990827No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC94330.1392359660685213No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA84030.12403263255314155No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA81290.11998825062769103No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT78550.1159438687022405No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC76700.11321317287666258No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC75720.1117666421150051No Hit
AAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATA74200.1095230433826384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72150.10649713719753855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)