FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629221
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98820.6065475463427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62800.3854602905314871No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC47320.29044555649601866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.27835388814654366No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA43400.26638497785137805No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC41320.25361814020320145No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT39660.24342922169552197No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA38240.23471339983955525No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC36650.22495413452195867No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT35460.21765003029054988No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC29270.17965641248179345No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG26490.16259304293278812No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC25360.15565721286430753No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA24570.1508082697190866No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA23540.144486229922153No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC22600.13871660136961161No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC21910.13448144849593763No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG21540.1322104244912139No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA20840.12791389259038521No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA20200.1239856348524847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.12140771571198751No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC19650.12060978835897647No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC19640.12054840933182177No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG19580.1201801351688936No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC18930.11619049840383841No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA18540.11379671634480527No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT18380.11281465191033016No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA18370.11275327288317546No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC17910.10992983763405946No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC17160.1053264105974573No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA16490.10121401577809272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.085.320731
GTATCAA75850.064.2273561
TTCTCTG8050.052.459567
CGTACTT350.007807828550.9818235
TATCAAC100400.048.639032
ATCAACG101800.047.7364123
CTAGTCG505.3426455E-447.583037
CAACGCA104150.046.657875
TCTCTGC10200.044.317538
CCCGTGC558.544452E-443.2573058
AACGCAG118800.041.805386
TCAACGC117700.041.6907464
TCAATGG758.2059E-539.6537443
ACGCAGA125000.038.875347
CGCAGAG125550.038.7050368
GGGGCGC554.7116737E-737.8553720-21
GCAGAGT128950.037.684519
GGCACGT401.1998186E-437.1776718-19
TAGCGTG650.001942533336.6023339
TCTAACG5600.035.051082
Produced by FastQC (version 0.11.5)