FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961904
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC463870.6662976105387262No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA411980.5917634026553655No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG400750.5756327579351855No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA382700.5497059425122783No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA341490.4905123655827486No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC325080.46694122757222734No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA323640.46487282789305917No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT301590.4332004578057957No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC300290.4313331525398799No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC284170.4081785672425245No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC258480.37127774241069683No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC240920.3460547574341732No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA233130.3348652897253395No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC226540.3253994884158127No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC220620.3168960675125655No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA206520.2966429873207099No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA205170.29470386262148973No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA205040.29451713209489816No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT193820.27840085126137903No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA187060.2686908638786171No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC163110.23428935532578443No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG158480.22763887580179218No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA155380.22318607093691611No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC152070.21843162445216138No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT148700.21359099464744127No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT144190.2071128817633797No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG137810.19794872207373154No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC132420.1902065871635116No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA125850.18076951362730656No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA123880.17793982795511115No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA123000.17667580592895277No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG122450.1758857921626038No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT121840.17500959507628946No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT121370.17433449240322763No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG119700.1719357233308589No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA113200.1625991970012801No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA110080.15811766436308228No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT108720.15616417577720118No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG108540.15590562581730516No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA106060.1523433819254043No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT105280.15122299876585485No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG104570.15020316281293164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104340.14987279341973114No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG103610.14882422969348616No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG102170.14675583001431794No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT100610.14451506369521902No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT99050.14227429737612007No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC95080.13657183437174658No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT94530.1357818206053976No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC94510.13575309283207582No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT94100.13516417347897933No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG91600.1315732018137567No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA91310.13115664910059088No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC91050.13078318804740774No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT90020.129303707721336No Hit
ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC88520.12714912472220244No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA87710.1259856499026703No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA87460.12562655273614803No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT86600.12439125848331146No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA85390.12265322819734373No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC84780.1217770311110294No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG84750.12173393945104673No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA84500.12137484228452447No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG84300.12108756455130665No Hit
ATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAG84030.12069973961146262No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT83500.11993845361843543No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA81720.11738168179279691No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT80040.11496854883376731No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT79580.11430781004736636No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG78830.11323051854779957No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC78740.11310124356785155No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA78250.1123974131214679No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA77750.11167921878842339No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC77030.11064501894883928No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA76950.11053010785555216No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT74990.10771478607001764No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA74040.10635021683723304No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA73800.10600548355737167No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG71530.10274488128534953No Hit
GGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGG71430.10260124241874063No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC71230.10231396468552281No Hit
CCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATA70170.10079139269946842No Hit
GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT69850.10033174832631993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAG38550.077.129376
AATACAG40550.076.027124
GTATCAA310150.073.4921341
ACAGAGA43650.068.116717
GGTATCA184600.065.623371
ATACAGA51150.059.5436745
TCTCTGC56250.057.46618
AGAGAAA54000.054.127779
TTCTCTG63150.051.643157
TATCAAC474550.048.2705882
CTCTGCA69450.048.0987329
ATCAACG478850.047.7294273
TCTCACA35950.047.68872
TCAACGC480350.047.4154324
AACGCAG492500.046.222116
ACTGTGC111950.045.7871678
ACGCAGA499700.045.512237
CGCAGAG500850.045.4221088
CTGTGCA109800.045.044559
CAACGCA506000.044.9937555
Produced by FastQC (version 0.11.5)