FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5891229
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196970.3343444975573009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119580.20297971781439833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117300.19910955761522764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC17500.045.5478253
TACGACA17550.044.0622984
ATATACG18100.042.3940241
GTATCAA331400.042.054581
ACGACAC19350.039.6560675
GATCTAC22150.034.6425251
ATCAACG418400.034.1351173
GGTATCA198550.034.0930521
TATCAAC422850.033.8881342
TCAACGC425250.033.5852624
CAACGCA427750.033.5141265
CGACACT23000.032.8458446
AACGCAG437900.032.7647556
TACACGA10500.031.7248545
CTACACG12150.029.8644454
TCTACAC38850.028.938213
ACGCAGA508550.028.1778437
CGCAGAG509300.028.1363478
GCAGAGT558350.025.6965839
GACACTC33650.024.9251987
Produced by FastQC (version 0.11.5)