FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7511038
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156850.2088259971524575No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG155740.20734817211682327No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG112180.14935352477247485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCAA66500.053.4741251
CCAACCA75700.046.904864
TCCCAAC81350.044.6701352
GTATCAA194300.043.5202061
CCAGGAC84650.040.9409688
GTCTCGC22050.040.7223171
TCTGTCG26200.038.3444028
CAACGCA221500.037.377045
AACGCAG226900.036.654866
TCTCGCT24750.036.2856862
CTCGCTC24600.035.7818763
ACCAGGA97950.035.5060777
TCAACGC234900.035.4980434
ACGCAGA235950.035.349967
CGCAGAG236950.035.1728748
ATCAACG239550.034.7590873
ACTTTTT128800.034.2629363
TATCAAC252400.033.0834922
CGCTCTG26200.032.6889235
GCAGAGT256400.032.5508779
Produced by FastQC (version 0.11.5)