FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004898111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223360
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157300.4880000992752904No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC106580.33064876402263477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.306295294351236No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC85640.26568549588007545No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA74380.23075300307753402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.21567556835103743No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC63910.19827136900625433No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA59500.18458999305072968No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC52360.16243919388464212No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT51160.1587163704953837No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC50960.1580958999305073No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT46300.14363893576888714No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC42330.13132259505609056No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA41700.12936811277672988No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA39700.12316340712796585No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT37380.11596594857539957No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA37310.11574878387769283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.11469398391740296No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC36810.11419760746550184No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG35840.11118832522585129No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT35150.1090477017770277No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA33070.10259480790231312No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT32840.10188126675270526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)