FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223360
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA87680.27201429564181473No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA79530.24673012012310136No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC79120.24545815546510472No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC76510.2373610145934677No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC71580.22206641516926434No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52460.16274942916708032No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT49260.15282190012905786No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC48320.1499056884741388No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA47310.14677231212151295No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC45430.14093988881167477No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG43060.13358731261788942No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA41740.12949220688970514No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG39930.12387694827757371No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC38610.11978184254938945No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC36460.11311178397696814No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA36290.1125843839968232No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG36290.1125843839968232No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC34210.10613149012210861No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG33390.10358756080611536No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT32470.1007333962076839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.080.612971
GTATCAA68150.062.8611641
ATCAACG91300.047.1173443
CAACGCA92350.046.5172925
TTCTCTG14850.044.0169227
TCAACGC104350.041.6803784
GTCGTGC2300.041.3337821
TATCAAC105100.041.043742
CTAGATA2850.039.611542
AACGCAG111000.039.2915846
GCTCTAG3150.037.7252771
ACGCAGA114000.037.582347
CGCAGAG113700.037.4741978
GTACTGG12250.036.862991
GCAGAGT116100.036.855389
TCTCTGC19250.035.809728
CTCTGCA19300.034.1795469
CGCGTGC350.002805177133.96223424-25
GATTCGA700.00280603733.95969
GCATAGA4200.033.952751
Produced by FastQC (version 0.11.5)