FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933361
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328580.8353670054693683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192490.48937791369772565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126690.3220909547839621No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT55850.14199052667680387No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41830.10634670959517828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA401850.056.3667641
GGTATCA200450.052.138181
TATCAAC500700.045.119842
ATCAACG508050.044.397973
TCAACGC518200.043.5627754
CAACGCA522150.043.2560165
AACGCAG534900.042.2810976
ATACGAC4050.041.124843
ACGCAGA596650.037.924697
CGCAGAG599350.037.743928
GCGCAGA8300.037.2662241
GCAGAGT626350.036.1263929
CATGGGT78550.034.0774844
GGGTTAG26900.033.1695987
GTCAACG12050.033.0733261
GGGGTTA26400.032.446316
TATAACG3300.032.445072
ATAGGGG28700.032.1255843
GATCTAC7100.030.997991
TCTGTCG12750.030.7917888
Produced by FastQC (version 0.11.5)