FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323234
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC68570.5182001067082618No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT48360.36546823917765114No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT38570.2914828367469397No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG36550.2762172072362107No Hit
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGG32920.2487844175708907No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT26950.2036676808485876No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT26130.1974707421363115No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG24520.18530358198172056No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT23930.18084480900581454No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC22990.17374100121369312No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA21910.16557917949508555No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC21520.16263185498558833No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGG17830.13474563078034574No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG17800.1345189135103844No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT17540.13255403050405296No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG17370.13126929930760547No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG17220.13013571295779885No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC16630.12567693998189283No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA16560.12514793301864977No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA16420.12408991909216359No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG15750.11902656672969406No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG15590.11781740795656702No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAA15480.11698611130004216No Hit
TGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGAC15040.11366092467394277No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG14830.11207390378421352No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG14610.11041131047116383No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14500.10958001381463899No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC14390.10874871715811413No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG14190.10723726869170533No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA13850.10466780629881034No Hit
GTTTTGGGATGCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTG13580.10262735086915843No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT13500.10202277148259492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107800.054.688761
GGTATCA66400.047.394811
TATAAGA4800.044.617422
GTTAAGG905.1620955E-639.659933
TATCAAC149200.039.513732
GTATAAG6100.038.034521
TTAAGGT1104.7070716E-737.8572044
ATCAACG156900.037.6124733
TCAACGC158400.037.0685124
CTATGCA650.001940623636.609164
TCCTAAC650.001940623636.609161
TCTATGC851.7123323E-434.9940533
ATAAGAG5800.034.8733863
CACTGTG30550.033.6882177
ACTGTGC30400.033.0716868
CTGTGCA30450.032.822019
CAACGCA183100.032.035525
CCCTGCA14550.031.4826249
TATACCC15800.030.87455
TCTATAC15400.030.1312453
Produced by FastQC (version 0.11.5)