FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379272
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209901.5218173065211213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156111.1318289648452227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80200.5814661647593803No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72310.5242620744856706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.46046030079636213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.30153588269753895No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG28810.2088783068169295No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT28560.2070657564280287No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA22220.1610994785655041No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT19580.14195894645871154No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG14540.10541793061847118No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC14160.10266285402734196No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA14050.1018653318562256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.086.346981
GTATCAA46250.081.665051
ATCAACG59700.062.8323333
TCAACGC60850.061.4082344
CAACGCA62200.060.184625
AACGCAG65000.057.9243166
ACGCAGA71850.052.101377
GTACTTT99250.051.720221
CGCAGAG72850.051.3861858
GCAGAGT76100.049.1916359
TATCAAC78550.048.2124672
ACTTTTT116250.044.2206423
CGTCAAT1002.8709728E-643.1940426
TGGTATC8450.042.6023062
TACTTTT126800.040.54142
GTGGTAT9150.039.844381
GGCTAGA5700.034.5387881
TCTGTCG3950.032.80568
ACGGGAC4850.032.6588363
GCTCTAG3350.032.648611
Produced by FastQC (version 0.11.5)