FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806200
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247660.515292746868628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129950.27037992592900834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124290.2586034705172486No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA91830.19106570679538928No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC63280.13166326827847363No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC56600.11776455411759809No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT55170.11478923057717115No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA50000.10403229162331987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA306900.063.391331
GGTATCA165750.053.703961
CAACGCA457050.042.705925
TATCAAC466900.042.085822
AACGCAG481200.040.5979926
ATCAACG482600.040.568933
TCAACGC484850.040.356164
ACGCAGA496650.039.298757
CGCAGAG498200.039.1756748
GCAGAGT523400.037.346259
AGAGTAC517500.031.00406310-11
GAGTACT137950.028.83458312-13
AGTACTT149150.028.62123312-13
CAGAGTA510250.027.89317710-11
TTTAGGG49950.026.8842832
ATTTAGG48100.026.4372791
TACGGGG120750.025.6851114-15
TACGGGT86000.025.51773614-15
GGGCTAT50650.025.4553266
GTACTTT157850.025.2620414-15
Produced by FastQC (version 0.11.5)