FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806200
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA95690.19909699970870962No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60540.1259622986975157No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC59490.12377762057342598No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT57510.11965794182514251No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA49240.10245100079064541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA262100.062.02361
GGTATCA146000.043.4865461
CAACGCA379800.042.1526685
TATCAAC388800.041.504742
ATCAACG402550.039.7154463
AACGCAG404300.039.549466
TCAACGC405400.039.4068874
ACGCAGA408450.039.065977
CGCAGAG408600.039.0370758
GCAGAGT431600.037.1516349
AGAGTAC415800.030.38914910-11
CAGAGTA414900.026.91428410-11
TTTAGGG49250.026.5797392
ATTTAGG47900.026.0871751
TATTCTC26700.026.0699925
TACGGGG124650.025.8613414-15
AGTACGG492850.025.41127812-13
TACGGGT96700.024.47939314-15
GGGCTAT50800.024.4644836
CTATTGA50550.024.1197179
Produced by FastQC (version 0.11.5)