FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004898175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205714
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC158740.4951782972529677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134910.4208422834975297No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA125880.3926738317891115No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC120470.375797716203005No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC100730.31422017060785834No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT93070.29032533781865755No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA75640.23595367521868763No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA75350.23504904055695547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72340.22565955665414944No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC63770.19892604268503056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.19596258431039074No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG62340.19446525797373065No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC60880.1899108903663895No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA60520.18878789561389445No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA57890.1805837950609443No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA55160.17206775152118997No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53290.16623441766795166No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC52010.16224154743685806No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA51240.1598395864384658No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT50070.15618985349285683No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT50050.15612746489549598No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49450.15425580697467084No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA49210.1535071438063408No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG48930.15263370344328908No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG47890.1493894963805255No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47050.14676917529137035No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC46990.14658200949928785No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT45690.1425267506708334No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC45340.14143495021701874No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44900.1400624010750803No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC44380.13844029754369855No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG44070.13747327428460554No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC43820.1366934168175951No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA43290.13504011898753288No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT40140.12521391490320097No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG39500.12321747978765417No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA38140.1189750551671172No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG37340.11647951127268372No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC36100.11261141823631178No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA35610.11108289760097126No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG35460.11061498312076498No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG35420.1104902059260433No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT35290.11008468004319787No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC35290.11008468004319787No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC35110.10952318266695034No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT34440.10743316465536226No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT33950.10590464402002175No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG33650.10496881505960919No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC33180.10350268302162952No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC33050.10309715713878406No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA32520.10144385930872185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)