FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004898178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648794
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45400.1713987573212564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42040.15871373915827353Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT39740.1500305422014698No Hit
TTCTCATTCTTCCTTCCTGTCTTGCTACCCTCACAGCCTCATCCAAAAGA35580.13432528161872911No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC29690.11208874680326217No Hit
ATATACGACACTCTTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA28200.10646354529646321No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT27340.10321678469522357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)