FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824738
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566400.8299219691657028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353120.5174118039403124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265440.3889380075835878No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144320.21146599327329488No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC131210.19225646464377094No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG129670.18999996776433029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83640.12255415519247773No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG82480.12085445624432761No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC81270.1190814944104814No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC78340.11478828930868848No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG72350.10601139560229272No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG71100.10417982345988959No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG70610.10346184718006757No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA68900.10095625648926011No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA68340.10013571216946351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.084.8288041
GTATCAA246150.059.9840771
GCTCTAG14700.048.9865761
ATCAACG321200.048.4683763
TCAACGC324750.048.011854
CAACGCA325650.047.8791585
AACGCAG340900.045.7292636
GCAGCGC16400.045.7123878
TGCAGCG17650.041.8007627
ACGCAGA382400.040.7964137
CGCAGAG385850.040.4624828
TATCAAC386850.040.305822
GCAGAGT409100.038.177469
TGGTATC32750.035.7984282
GTGGTAT34600.033.1963421
TGGTCCT33200.032.623435
CTAGCCT57900.030.012394
GTCGTGC8450.029.5802351
CGTCAAT5650.028.4338236
CAGAGTA431250.028.40759310-11
Produced by FastQC (version 0.11.5)