FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309669
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450430.6162112128469839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280000.38305428057002305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233430.31934414540521605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144060.19708142735327688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119800.072.147431
GTATCAA268800.057.080651
TCTGTCG46100.054.4580158
GTCTCGC41350.050.8872341
TCTCGCT44250.048.808352
CTCGCTC42700.048.071983
TGCGGAC3850.047.9017339
CGCTCTG42150.046.2957765
ATCAACG354500.043.8051383
CAACGCA356150.043.5652055
TCAACGC360500.043.076064
AACGCAG370550.042.1116036
TATCAAC373400.041.396692
ACGGGAC16850.040.95993
GAGTCGG8350.039.9770931
GTACGGG315900.039.3051341
ACGCAGA397700.039.2367447
CGCAGAG401100.038.9486438
TCGCTCT58600.038.379124
GTCTCAC63050.037.6276971
Produced by FastQC (version 0.11.5)