FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309669
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218580.29902858802498444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC43750.059.282321
TCTCGCT45100.057.1130032
CTCGCTC43750.056.297853
TCTGTCG49150.054.944168
CGCTCTG44700.052.97775
GGTATCA78500.052.92371
TGCGGAC5150.044.946319
GTATCAA156000.044.7789271
TCGCTCT60500.043.066234
ACGGGAC17400.042.9776953
GTCTCAC70400.042.5736161
GGCTAGA36050.040.170421
GTACGGG351300.039.7188151
CAACGCA172800.039.3612255
ACGCAGA177350.038.451797
AACGCAG179250.038.0773246
ATCAACG179600.038.00263
CGCAGAG179150.037.9997148
TCTGTCA74900.037.4811638
TCAACGC182600.037.3787544
Produced by FastQC (version 0.11.5)