FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5412501
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394260.7284248076813289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208760.38569969779220364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193030.35663734750349235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA322150.064.2075651
GGTATCA150300.061.674541
TATCAAC459850.045.1830562
CAACGCA466600.044.3507425
ATCAACG467300.044.297433
TCAACGC469000.044.1115264
AACGCAG475750.043.619826
ACGCAGA504300.041.12687
CGCAGAG516200.040.1319278
GCAGAGT538350.038.5359049
AGAGTAC523850.032.61654310-11
AGTACTT232850.029.02225912-13
CAGAGTA533250.028.15894110-11
AGTACGG377700.027.36109512-13
GAGTACT232400.026.01083812-13
TACGGGT71550.025.5208214-15
GTACTTT249550.024.19214214-15
TACGGGC108750.023.68233514-15
GAGTACG382150.023.52128812-13
TACTTTT289300.023.42781414-15
Produced by FastQC (version 0.11.5)