FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5412501
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70810.13082676566711027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA224200.060.288271
GGTATCA115450.045.5016141
CAACGCA305150.043.584295
TATCAAC313450.042.7804872
TCAACGC311850.042.673694
AACGCAG313100.042.548976
ATCAACG316400.041.9675643
ACGCAGA321450.041.3754927
CGCAGAG331700.040.1138
GCAGAGT346300.038.4260989
AGAGTAC330500.031.79053710-11
AGTACGG378750.027.44843912-13
CAGAGTA341850.026.89098710-11
TACGGGT71300.025.10638414-15
GAGTACG384150.023.71169512-13
TACGGGC105450.023.2922114-15
GTACGGG388900.022.6477114-15
TACGGGA155100.022.04769714-15
TACGGGG112600.021.97162414-15
ACGGGAT32200.019.12240616-17
Produced by FastQC (version 0.11.5)