FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469408
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118370.2648449190586315No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA65000.14543313118873907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61390.13735599882579527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55080.12323779793654999No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC54780.12256656810029427No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC51280.11473555334397754No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT46240.10345889209488147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA276950.067.088761
GGTATCA183150.048.8542561
TATCAAC410950.045.390412
CAACGCA417000.044.8359575
ATCAACG428400.043.4584773
TCAACGC433050.042.907744
AACGCAG446750.041.9791566
CGCAGAG444200.041.814968
ACGCAGA453050.041.205187
GCAGAGT463900.040.1323629
ATTTAGG35550.035.048341
ATTCTCT20650.029.6339426
TCTCTGC20800.029.4202358
TTTAGGG41200.029.356062
GCTATTG44450.027.209978
CTATTGA43950.027.0281059
TTCTCTG24150.026.8300087
GGCTATT46550.026.6013817
ACTGTGC22500.026.5574348
GGGCTAT51000.024.985826
Produced by FastQC (version 0.11.5)