FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8032622
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365860.4554677165189648No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG134540.16749200945843087No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT114150.1421080190254191No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA107600.1339537700143241No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87060.10838304105434066No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC80690.10045287827561161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.062.7846371
GTATCAA207650.055.2947351
ATCAACG233950.047.8150063
TCAACGC239550.046.6233254
CAACGCA242600.046.159745
AACGCAG260700.043.0284846
ACGCAGA260950.042.94177
CGCAGAG261250.042.776228
TCTGTCG11150.038.3847278
GCAGAGT299150.037.1375279
TATCAAC326350.034.8957672
GTCTCGC11950.033.8356481
GTACTTT117550.033.5875931
GCTCTAG12600.033.033981
TCTCGCT12800.032.057282
CGGGTCA8350.030.6256874
GTACGAG5450.029.4578271
CGTTACG1251.2739303E-528.5459885
TAGCGTG8550.028.5043079
ACGGGTC11100.027.3240853
Produced by FastQC (version 0.11.5)