FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004898225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564411
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224691.4362593973067181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127100.8124463456214511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113590.726087965374828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69140.44195547078101594No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.33533387325964853No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41100.26271868454006014No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.14849039031303155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22770.14554998654445667No Hit
GCCCAGGAGAGGGCAGCTCTGAACTGCTGGCTGGGACCAGCTCAGTAGGA21770.1391578044388591No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.11627379250081979Clontech SMART CDS Primer II A (100% over 21bp)
GCTCTGAACTGCTGGCTGGGACCAGCTCAGTAGGAACAGATGAAAGGAAG17030.10885886125832661No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16600.10611022295291966No Hit
CGGGTACACAGGGCCACGCAGTGACCACCGCGGGACCAGGGCTGGTGAGC16160.10329766282645672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.1013160863737215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)