FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991354
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331310.5529801777694991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162500.2712241673584969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158270.26416399364818033No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC77790.12983709525426138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA329750.072.263061
GGTATCA141250.066.9327241
TATCAAC482700.049.7302932
ATCAACG498750.048.070383
TCAACGC502400.047.6616134
AACGCAG508000.047.1435746
CAACGCA514750.046.4942365
ACGCAGA529550.045.225077
CGCAGAG542350.044.1573458
GCAGAGT571050.042.0108959
AGAGTAC555450.034.59113710-11
CAGAGTA559400.029.82783710-11
AGTACGG458850.028.93206412-13
AGTACTT219600.026.98673812-13
GAGTACT201000.026.21786712-13
TACGGGC115250.025.90545814-15
GAGTACG463400.024.59021812-13
TACGGGT104500.024.44417614-15
TACGGGA168400.023.78614414-15
GTACTTT231950.022.83326714-15
Produced by FastQC (version 0.11.5)