FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004898244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30541
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG5661.8532464555842965No Hit
TCTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGT910.2979601191840477No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC900.2946858321600472No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT510.1669886382240267No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC390.12769719393602041No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGG360.11787433286401885No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT360.11787433286401885No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT340.11132575881601782No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC330.10805147179201728No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC330.10805147179201728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10477718476801677No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA310.10150289774401625No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC310.10150289774401625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)