FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551204
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA283930.4334012496023632No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA240210.3666654251645957No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC220130.3360145707567647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216130.32990882286675854No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC166050.25346485928388124No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC159350.2432377315681209No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC152330.2325221440211601No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC149820.2286907872201812No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC139940.21360958993186596No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC139740.21330430253736565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137660.21012931363456241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131750.20110807112707832No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG127100.19401013920494614No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT124260.18967505820304176No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC111430.17009087184584698No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC106430.16245868698333926No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA105560.16113068681726292No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA93850.14325610986926982No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA93750.14310346617201966No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA93120.1421418108793437No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT86730.13238787862505885No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA84060.12831229190847973No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT81920.12504571678732643No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG80000.12211495780012345No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT79530.12139753242304772No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC76870.11733721007619363No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG75640.11545969260001673No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT75380.11506281898716632No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG72830.11117040470728738No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA71740.10950658840726071No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC70140.10706428925125824No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA69850.1066216225292328No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA68710.10488148438058104No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG68480.10453040387690567No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG68100.1039503578273551No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA67600.10318713934110432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82350.087.624841
AAGTACG5100.075.880711
GTATCAA209550.061.9948041
ATCAACG307150.044.3749353
CAACGCA317650.042.8870935
TATCAAC319000.042.7638242
TCTCTGC52650.042.2539028
TCAACGC323000.042.2516944
AACGCAG328300.041.6045656
CTCTGCA53300.041.1806079
TTCTCTG53050.041.1504177
ACGCAGA348900.039.1439367
CGCAGAG351300.038.859588
GCAGAGT362700.037.588999
ATTCTCT72050.032.4487956
AGAGTAC375950.031.20904710-11
TCTATAC66100.030.8713673
ATTTAGG107200.028.935511
TATTCTC85700.028.877035
CAGAGTA375150.027.95380810-11
Produced by FastQC (version 0.11.5)