FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551204
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA272410.41581669567914537No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA251850.3844331515245137No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC208720.31859792490052213No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC175550.2679660105226459No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC172590.2634477570840414No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC159550.2435430189626212No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC146500.2236230164714761No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC139910.2135637968226909No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC137960.21058724472631288No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA137710.2102056354831875No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG124550.1901177249250672No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT122770.18740066711401446No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC109290.16682429672469365No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA105480.1610085718594628No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT105200.16058116950716234No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC103990.15873418077043547No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA94760.14464516751424625No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT92860.14174493726649331No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC90790.13858521273341512No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA90080.13750144248293902No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG89680.1368908676939384No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA85760.13090723476173236No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT83520.1274880159433289No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC82460.12586999275247726No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG81760.12480148687172618No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG76870.11733721007619363No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG76280.11643661226241771No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA70220.10718640420905837No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT69300.10578208219435695No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA69180.10559890975765675No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA66220.10108065631905219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.080.033751
GTATCAA146000.060.2083361
ATCAACG188700.046.1428343
CAACGCA191200.045.348365
AACGCAG197750.044.1994066
TCTCTGC52500.044.1669778
ACGCAGA196850.043.9182057
CTCTGCA51900.043.8743329
CGCAGAG198150.043.657748
TCAACGC202650.042.96584
TATCAAC206350.042.7436642
TTCTCTG53850.042.3995367
GCAGAGT205950.041.974139
GCTCTAG4300.034.5785371
ATTCTCT70400.034.290126
TCTATAC60750.033.580173
AGAGTAC220950.031.14820310-11
CAGAGTA217000.030.37375610-11
ATTTAGG107650.030.1657161
TATTCTC81950.029.7527315
Produced by FastQC (version 0.11.5)