FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433242
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219060.6380558084749051No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA123160.3587279894630207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102990.2999788538064022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95950.27947345395401785No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT90820.26453130889112975No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC84100.24495797266839917No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC83540.24332686131650494No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC77860.2267827318901493No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC70270.20467534767429735No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT63610.18527677338212686No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA62860.18309224925012568No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT62310.18149026488665815No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC58330.16989772349283855No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT56230.16378105592323525No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC54470.15865470596013914No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC51630.15038264124696132No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA49470.14409121174679793No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA47950.1396639095059422No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC45100.1313627178043377No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT44590.1298772413945769No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC40790.1188089857924376No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG40630.11834295397761066No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA39470.1149642233201155No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG38670.11263406424598091No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA35980.10479890435920335No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT35700.10398334868325623No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA35470.10331342794944254No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC35280.10276001516933558No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG34780.10130366574800145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA265050.068.5473251
GGTATCA130500.052.8350641
TATCAAC377700.048.6019822
ATCAACG401050.045.7870983
TCTCTGC21300.044.124778
TCAACGC422700.043.4278874
CAACGCA432600.042.4340445
AACGCAG456600.040.425086
ACGCAGA464000.039.5752537
CGCAGAG467250.039.312718
GCAGAGT478000.038.4783679
CTATTGA51050.035.306279
GCTATTG51450.035.2630168
TAACCAC4400.035.150035
TTCTCTG27800.033.5938577
GGGCTAT54850.033.510966
TTTAGGG54800.032.998792
GGCTATT56000.032.7165647
AGGGCTA63600.029.2746855
AGAGTAC487150.029.14705810-11
Produced by FastQC (version 0.11.5)