FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433242
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA127180.370437038810547No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC99480.28975528086863667No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT90780.264414800937423No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC79330.23106439918887164No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC76650.22325836629052076No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC68400.19922860083850777No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT63960.18629621797706075No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA60650.17665518480782888No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT58250.1696647075854251No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT57070.16622772295107657No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC56950.16587819908995638No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA55480.16159653179123407No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC54300.15815954715688552No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA49170.14321740209399747No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC48710.14187756062637005No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC43820.12763446328572237No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG42190.12288676417217313No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA41110.11974104942209143No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG40440.11778954119750369No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT39580.11528462019280901No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT39530.1151389852506756No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC39440.11487684235483547No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA38790.11298358810710112No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG36390.10599311088469732No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC35890.10453676146336319No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC35740.10409985663696296No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA35640.10380858675269614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA235650.067.0212551
TATCAAC330000.047.823192
GGTATCA120900.045.3498761
ATCAACG350200.044.79273
TCTCTGC22300.042.4297458
TCAACGC369300.042.3954474
CAACGCA379550.041.2312365
ACGCAGA393050.039.9548727
AACGCAG397950.039.7021686
CGCAGAG396450.039.507148
GCAGAGT398350.039.213579
TAACCAC4100.033.3924875
GTCTAAA1806.002665E-1033.0727421
TTCTCTG31450.030.2745387
CTATTGA52800.030.0918739
GCTATTG53250.029.8380098
TTTAGGG55400.028.583452
AGAGTAC409750.028.55199210-11
GGGCTAT57200.028.0896246
ACTGTGC60400.027.7837168
Produced by FastQC (version 0.11.5)