FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5350833
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300810.562174151202252No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT230650.43105437975732No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT195160.36472825819830296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182430.34093756990733964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA180490.33731196619292736No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA173630.32449153243990236No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC171800.32107150419383296No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC169640.31703474954273475No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT160660.30025231585437256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139550.2608005146114633No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA134580.2515122411781493No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG128150.2394954206195559No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC127610.23848623195678129No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA123430.2306743641597486No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC119780.22385299634655015No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT119510.22334840201516287No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG111750.20884598715751362No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG110540.20658465700574097No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG108310.20241708160206084No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT107570.2010341193604809No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC99690.1863074403555484No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT99640.18621399696084703No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA99290.18555989319793761No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC98930.18488710075608789No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT97030.18133625175743665No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC96510.18036444045254263No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG94350.1763276858014444No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC94160.17597260090157926No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA93880.1754493178912517No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA90540.16920729912520163No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT88080.16460988410589528No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG87970.16440430863755232No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT87660.16382495959040397No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC87480.16348856336947912No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG86980.1625541294224656No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT81410.1521445352527354No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC80660.1507428843322152No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC80540.15051862018493195No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC79950.14941598812745605No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC79750.14904221454865063No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA79590.14874319568560634No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC79440.14846286550150228No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG79300.1482012239963385No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA79280.14816384663845797No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG79050.14773400702283176No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA78610.1469117051494599No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG77770.14534185611847728No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC77260.14438873349252349No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC77080.14405233727159866No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG76220.14244511088273545No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA74960.14009033733626147No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA73530.13741785624780292No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG73330.13704408266899754No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT70990.13267093179697442No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA70510.13177387520784148No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC69880.1305964884346045No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG69130.12919483751408425No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG68010.12710170547277405No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA66670.12459742249477791No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG63950.11951410182302458No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG63950.11951410182302458No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA63840.1193085263546816No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG63750.11914032824421918No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG63420.11852360183919027No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG61660.11523439434570282No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC61420.11478586605113633No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGG61330.1146176679406739No Hit
CCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAG60840.11370192267260068No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA60570.11319732834121342No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTC60550.11315995098333288No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC60440.1129543755149899No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA60390.11286093212028855No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT60340.1127674887255872No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC60010.11215076232055832No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA59510.11121632837354484No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAA58320.10899237557965274No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCG58060.10850646992720574No Hit
GTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG57670.1077776114485352No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGG57370.10721695108032712No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA57250.1069926869330439No Hit
CTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCG57240.1069739982541036No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT57170.10684317750152172No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC57090.10669366806999955No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG56560.10570316808616528No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC56430.10546021525994177No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGG56420.1054415265810015No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT55580.10387167755001885No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC55550.10381561151319804No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC55400.10353528132909401No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC55350.10344183793439266No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATG55050.10288117756618456No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG55030.10284380020830401No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC54460.10177854550870866No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG54080.10106837570897839No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCA54050.10101230967215757No Hit
CCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT54010.10093755495639652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134050.079.132691
GTATCAA301950.067.973411
CTCTAAT3600.052.9427261
TATCAAC403500.051.5165632
ATCAACG425800.048.650873
TCAACGC446800.046.5772824
CAACGCA446250.046.55475
AACGCAG452150.046.0919536
GACCGTT15550.043.9982037
TAGGACC16050.043.7395674
ACGCAGA484600.042.845937
CGCAGAG489200.042.443048
GCAGAGT503350.041.261729
CTATCAA6700.040.892331
CGTTAAC2200.040.6094781
TTAGGAC17500.040.1154333
TCTCTGC18700.040.0863468
ACTGGTT78450.040.0413323
TACTGGT79200.040.037742
GTACTGG81350.039.0237051
Produced by FastQC (version 0.11.5)