FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4792932
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT138350.28865420999087826No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG106720.22266120195320946No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA92050.19205363230690525No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT91560.19103129357979626No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT89090.1858778718329407No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA80470.16789305585808437No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT72120.15047156938592077No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC71190.1485312122099792No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT69820.14567283658520505No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA65740.13716030187784847No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA65050.13572068203763374No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA64330.13421847003045317No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT62470.13033775567857003No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC60080.12535124637695672No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC59920.12501742148647216No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51750.10797148801610372No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC50690.10575989811664342No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA50480.10532175294788243No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA50130.10459151099994743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64600.056.2763181
GTATCAA121000.053.7466961
ATCAACG153750.042.2208143
TCAACGC158100.041.0215074
CAACGCA159000.040.789315
AACGCAG160600.040.382946
ACGCAGA164400.039.558097
CGCAGAG165200.039.5105938
GCAGAGT170350.038.211339
TATCAAC174100.037.832572
GTACTGG30100.033.4069751
ACGGGTT13600.031.5000025
TACGGGT32450.029.3374424
TGGTTCA34150.028.748175
AGAGTAC171650.028.70142710-11
CAGAGTA174750.028.5668110-11
GGTTCAC40600.028.4310346
CGGGTTC12450.028.1967876
CTGGTTC35450.028.0296174
CTCTGCA18050.028.019399
Produced by FastQC (version 0.11.5)