FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004912221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982224
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA329130.8264979569205549No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA226410.5685516435037306No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC215940.5422598025625881No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC194980.48962589748843866No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC174700.4386995809376871No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC150060.37682460856044264No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC147460.3702955936180386No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC139910.3513363386891345No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC135610.34053835243823555No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT131020.32901212990529916No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC125540.315250975334386No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC117290.29453390869021934No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA116730.2931276593180092No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA116480.29249986941970113No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG116150.2916711867539345No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA114890.28850712566646175No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT111000.278738714848788No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT108480.27241059267384254No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT108020.2712554592609557No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC103720.2604574730100567No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG101810.25566115818698293No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT101000.2536271189164648No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT94010.23607411335977083No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA91420.2295702100132991No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA89740.2253514618966688No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT88950.22336764581801527No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA88920.22329231103021827No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA86920.21826999184375362No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG86890.21819465705595667No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG86470.21713997002679908No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA84460.21209253924440213No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA79700.20013941958061626No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG78770.1978040411589102No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC77440.1944641988999112No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC76640.19245527122532535No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC72960.1832142039222304No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA71380.1792465717649233No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA71280.17899545580560008No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC70640.1773883136659314No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG70550.17716230930254048No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT66510.1670172245458819No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA65510.16450606495264958No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT64570.1621455749350112No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC64480.1619195705716203No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA62220.15624434989091524No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG61740.15503899328616372No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT56190.1411020575437243No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT56030.14070027200880714No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG55640.13972091976744652No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC54680.13731020655794351No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG54090.13582862239793644No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA54060.13575328761013947No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC52870.132765007694193No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA52510.13186099024062936No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT51860.13022873650502834No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG51560.12947538862705865No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC51300.12882248713281824No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG50500.1268135594582324No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT49260.1236997215626243No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC48970.12297148528058693No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC48650.12216791421075258No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT47970.12046032568735461No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT47690.11975720100124955No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA46960.11792405449818995No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC46800.1175222689632728No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG46780.11747204577140814No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC46210.11604068480326572No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA45210.11352952521003339No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT45100.11325329765477783No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC44870.1126757309483344No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT44480.11169637870697378No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC44290.11121925838425965No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG44220.11104347721273339No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT44150.11086769604120714No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT43870.11016457135510209No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT43750.10986323220391418No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA43600.10948655826492934No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT43560.10938611188120007No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC43110.10825609006424551No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA42730.10730184941881722No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG42670.1071511798432233No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT42560.10687495228796771No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT42540.10682472909610309No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG42400.10647316675305056No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA41920.10526781014829904No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG41710.10474046663372025No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG41610.10448935067439702No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT41470.1041377883313445No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG41150.10333421726151015No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC40460.10160151714217985No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC40130.10077283447641318No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA39970.10037104894149601No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA39850.10006970979030813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)