FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5906183
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT267610.4531014362406312No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG208650.353273848778475No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT176920.2995504880224673No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA172460.29199907960860677No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA152650.2584579583802263No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT151990.25734048538624693No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC142180.2407307731575537No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA124620.21099921895410284No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC112790.19096936210747278No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC108730.18409521005359974No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC99990.1692971585878731No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC95480.1616610931290141No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA94480.1599679522290454No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC92870.15724199538009573No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC85540.14483127258332495No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC83060.14063228315140253No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA82370.1394640159304241No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA81940.13873596534343755No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG79980.13541740917949885No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT77580.1313538710195739No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT76230.12906813080461613No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG73600.12461517023769836No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC73110.12378553119671368No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT72980.12356542287971775No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT71390.12087332884876748No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG71210.12056856348677311No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC69390.11748704704883002No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC67620.11449018765588538No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA66640.11283090957391602No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA65870.11152719108094009No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA65340.11062982640395666No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC62370.10560119793104955No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT61610.10431441084707332No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA60990.1032646634890927No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT60610.10262126994710459No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT59270.10035246114114649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58750.069.508461
GTATCAA126050.058.7487951
ATCAACG161300.045.2457243
CAACGCA167150.043.626585
TCAACGC169300.043.107344
AACGCAG170150.043.0115246
ACGCAGA172700.042.2027477
CGCAGAG173550.041.929628
GCAGAGT177400.040.9179849
CTCTGCA37800.038.0918059
TATCAAC198700.037.2986342
TCTCTGC43350.034.313898
TTCTCTG44100.033.054037
ACGGGTT12850.031.0379565
AGAGTAC183350.031.00008810-11
ATTCTCT50250.030.90416
CAGAGTA182100.030.62457810-11
ATTTAGG78150.030.3924261
TATTCTC52500.030.3876385
TACGGAA6050.027.5501582
Produced by FastQC (version 0.11.5)