FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868426
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.36356805139727233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.26380493527707277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.24485850657184174No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT21690.11608701655832236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG2350.060.7342531
GGTATCA43950.057.2363361
GTATCAA76850.049.757371
ATACGGG3250.038.4260981
TATCAAC104650.037.2722132
ATCAACG110050.035.8215263
CAACGCA112300.034.9969865
TCAACGC112850.034.9327284
AACGCAG114950.034.187446
ACGCAGA125950.031.2454937
CGCAGAG127100.030.961138
GTCTCGC6400.029.734481
TCTGTCG7200.029.7217468
CTCGCTC6300.029.2578013
GCAGAGT135050.029.1825529
CGTCAGA5300.029.1687662
ACGGGTT3800.028.1642285
GTACTGG11850.027.099781
ACGTGCG1556.884955E-626.8454519
TCTCGCT6900.026.7136462
Produced by FastQC (version 0.11.5)