FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868426
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.049.522451
GTATCAA53150.046.3860631
TATCAAC65600.037.4010542
ATCAACG68750.035.3409233
CAACGCA68600.035.3313945
AACGCAG69900.035.0711256
TCAACGC71950.033.6863564
ACGCAGA73450.033.130377
CGCAGAG74350.032.8067178
CTCGCTC6750.031.7606373
GCAGAGT78900.030.6894369
GTCTCGC7000.030.6263281
TCTGTCG7800.028.220658
TCTCGCT8250.028.1514722
GCATCGT3150.026.440978
AGAGTAC74500.024.52115210-11
CAGAGTA79800.024.4212110-11
TCTGTCA17400.023.9336368
GTCTCAC14700.023.4963871
TCGCTCT8900.023.4190064
Produced by FastQC (version 0.11.5)