FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807283
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256470.5335030203131373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155320.3230931068547452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82960.17257149204654687No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA82830.17230106902381243No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC82060.1706993326583852No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT75910.15790624350594712No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT70910.14750535801616008No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT61190.12728603662401403No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA56740.11802924853810354No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC55970.11642751217267634No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA52680.10958372952039645No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG49430.10282315395203487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.074.444281
GTATCAA225100.063.956681
ATCAACG287150.050.341183
CAACGCA301250.048.2800335
TCAACGC305750.047.6666954
TATCAAC302650.047.5468672
AACGCAG310000.047.047646
ACGCAGA334800.043.472037
CGCAGAG342400.042.505798
GCGTAAG3550.041.8945661
GCAGAGT355850.040.9660349
GCGCAGA15000.040.851
GTACTGG33100.034.5079571
GTCAACG17700.033.610331
TACGGGT33650.031.2770924
ACGGGTT12700.030.9014175
CAGAGTA362050.030.15732410-11
AGAGTAC353150.029.57896410-11
GTCTAAT12050.029.1280231
CGTACTT3900.028.9691812
Produced by FastQC (version 0.11.5)