FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004912287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807283
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA70420.14648607123816093No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC68910.14334500382024523No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT68780.1430745807975108No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT65850.13697966190049554No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT65780.13683404950363853No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC57910.12046305574271371No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT54770.11393129965512744No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA52890.1100205667109675No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT49440.10284395572301443No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48400.10068057154113871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)