FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3806086
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG51140.13436375320999053No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT45870.12051750801216787No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA44490.11689173602488226No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41760.1097190131804694No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC41010.1077484849265098No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT40140.10546267215191671No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT38310.10065458321225532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.055.852931
GTATCAA111300.047.5833631
ATCAACG130950.039.8062323
CAACGCA133200.039.0438655
AACGCAG140550.037.516546
TCAACGC139650.037.369024
ACGCAGA143200.036.3636747
CGCAGAG143450.036.3327068
GCAGAGT148550.035.0848779
TATCAAC150500.034.912472
GCTCTAG3950.031.6716521
GTACTGG19700.031.4496361
CGGGACG2353.3651304E-1027.8851744
GGTTCAC24050.027.4680486
TAGCGTG2205.16593E-927.0436829
ACGGGTT12050.026.201835
CTCTGCA11800.025.7144179
ACTGTGC9350.025.453218
CAGAGTA159500.025.41009510-11
GTTCACT26050.025.1297257
Produced by FastQC (version 0.11.5)