FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585601
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208700.4551202775819353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124210.27086961992550157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94060.20512033210041605No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG84180.1835746284947164No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC52580.11466326878417898No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA52540.11457603921492515No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT51630.11259156651440018No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA50240.10956033898282909No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT48780.10637645970506375No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA47900.10445740918147917No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG46930.10234209212707342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77200.083.39441
GTATCAA197950.062.913041
ATCAACG252100.049.7565963
CAACGCA259750.048.5420075
TCAACGC263150.047.8475464
AACGCAG269400.046.817126
TATCAAC273000.046.012752
GCTCTAG3550.045.2545621
ACGCAGA285550.044.1421787
CGCAGAG289000.043.654948
GCAGAGT297500.042.3876729
CGGGTTC13900.032.085456
ACTGTGC12250.032.032718
ACGGGTT15600.032.025225
TAGATGG9000.031.7101867
CAGAGTA304750.030.71742610-11
AGCCTTA3950.030.1088776
AGAGTAC310750.029.38772610-11
CTTGTAT1653.423229E-728.837192
CGCATGT1454.0696523E-628.7247981
Produced by FastQC (version 0.11.5)