FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585601
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG86900.18950623920397783No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG58070.12663552716426918No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT56920.12412767704822115No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA54970.11987523554709623No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC51420.11213361127581752No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT51010.11123950819096559No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA46080.10048846378042922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.074.631181
GTATCAA129300.056.9092031
ATCAACG158750.046.089153
CAACGCA160750.045.5522965
TCAACGC167250.043.782434
AACGCAG168550.043.6120266
ACGCAGA171300.042.6255467
CGCAGAG172000.042.4432648
GCAGAGT176650.041.259119
TATCAAC187600.039.255432
GCTCTAG3800.037.630651
CGGGTTC15850.034.923826
ACGGGTT17650.033.419495
TACGGGT35850.030.4141754
CAGAGTA187500.029.3821510-11
TACTAGA7950.028.4793821
GTCGTGC3350.028.457011
AGAGTAC191500.028.05348810-11
AGCCTTA4700.027.860766
TAGATGG7800.027.4656967
Produced by FastQC (version 0.11.5)