FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004912401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC40.23460410557184752No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA30.17595307917888564No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATA30.17595307917888564No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA20.11730205278592376No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA20.11730205278592376No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG20.11730205278592376No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG20.11730205278592376No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG20.11730205278592376No Hit
GCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACAC20.11730205278592376No Hit
GAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC20.11730205278592376No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG20.11730205278592376No Hit
CCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT20.11730205278592376No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC20.11730205278592376No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA20.11730205278592376No Hit
CGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC20.11730205278592376No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG20.11730205278592376No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGG20.11730205278592376No Hit
CCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGT20.11730205278592376No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA20.11730205278592376No Hit
CATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAG20.11730205278592376No Hit
CCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT20.11730205278592376No Hit
TTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC20.11730205278592376No Hit
GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT20.11730205278592376No Hit
GTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAG20.11730205278592376No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20.11730205278592376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11730205278592376No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC20.11730205278592376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)