FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877716
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.3801913147305051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.3313144570681177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.21521767861130478No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA18000.20507772445756942No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC15170.17283494889007378No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14970.1705563075072119No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA13750.15665659507175442No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA13410.15278290472088923No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT12140.13831353193971627No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG11060.1260088684722621No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT10610.12088192536082287No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC9890.11267881638252009No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC9750.11108376741451678No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG9700.1105141070688013No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC9450.10766580534022395No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC9260.10550109602650516No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA9200.10481750361164659No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG8910.10151347360649685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG900.079.327791
GGTATCA29600.054.8728521
GTATCAA53450.043.9679261
GGGACCG558.5326406E-443.2672427
ACCTAGT905.156564E-639.6638951
ACGTCAG600.001308792939.6638951
TATCAAC71300.037.4665262
CTGTGCG2250.037.0175259
CTTAGAA650.001939315736.6128232
ATACGGG5400.036.358571
ATCAACG74250.035.81773
TGCACAC1001.0624111E-535.6975025
GACATAT1001.06282605E-535.695479
ACTGTGC3550.035.192728
CAACGCA77250.034.6577725
GGTTCAC5600.033.9976236
CTCTATG700.00278958533.9976232
TCAACGC80400.033.447914
TCTCTGC5700.033.3992738
AACGCAG80900.033.2411846
Produced by FastQC (version 0.11.5)