FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877716
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA16240.18502568028838487No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14560.16588509267234505No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13830.15756805162489917No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC12670.14435193160430027No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA12440.14173149401400909No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11950.13614882262599748No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA11810.13455377365799415No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC11770.13409804538142178No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC10970.12498347984997425No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA10400.11848935190881789No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT10310.11746396328653004No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC10020.11415993328138031No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC9930.11313454465909245No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT9750.11108376741451678No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9730.11085590327623057No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA9540.10869119396251178No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG9170.10447570740421731No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC9100.10367818292021565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.040.5634961
GTATCAA42800.039.9369281
ACGGTAC2600.038.9414523
TTCTCTG4000.038.6991357
ATTGCCT957.4528944E-637.591688
CTGTCGA1451.0918484E-732.8461959
ATCAACG52850.032.1171043
TCTCTGC4850.031.9077118
TATCAAC52900.031.8615782
CCTCAGG2450.031.6019422
ATTCTCT5300.031.4571656
CTCTGCA4950.031.2702459
GCTACCG4200.031.1861137
CAACGCA54600.031.0877135
ACTGGTT7000.030.6295763
TTCAAGA2750.030.3201872
TATTCTC5700.030.301195
GTACTGG7500.030.1758021
ACGCAGA56550.029.9001677
TACATGC1403.0701049E-629.7787532
Produced by FastQC (version 0.11.5)