FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004912432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT30.29469548133595286No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG20.19646365422396855No Hit
CAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTT20.19646365422396855No Hit
TCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCACCAATTC20.19646365422396855No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT20.19646365422396855No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT20.19646365422396855No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC20.19646365422396855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)