FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067824
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68490.6413978333508144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49920.4674927703441766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30670.28721961671586327No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT11220.10507349525764545No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC10830.10142120798933157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.067.757011
GTATCAA70000.055.992031
ATCAACG88650.044.0763053
TATCAAC90250.043.3607942
CAACGCA92300.042.3333055
TCAACGC93600.041.872424
AACGCAG95300.041.0006756
ACGCAGA101350.038.6118557
CGCAGAG102550.038.1600348
GCAGAGT106400.036.7233479
GTACATG6500.029.2802961
CAGAGTA108500.028.99642410-11
GTACTGG4800.028.4987261
TACATGC5900.028.2243882
GAGTACT55900.027.8745612-13
TGATCGC1954.8048605E-827.4489929
AGAGTAC108200.027.26295510-11
GTCTACC3150.026.4336011
TAAGACT6150.026.1100189
TCTGTCG1608.771638E-626.0193568
Produced by FastQC (version 0.11.5)