FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759316
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA43400.24668678054425697No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC40790.23185146954839267No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC37420.2126962978794031No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA36920.20985428427866284No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC29370.16693987890748452No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT28620.16267685850637406No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT27010.15352557471199035No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC23980.1363029722915042No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC23800.1352798473952377No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA23190.13181259080233454No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22980.1306189450900236No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA22240.126412764960928No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG21710.12340023054414329No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC21640.12300234864003966No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT21110.11998981422325496No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19850.11282793994938942No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA19050.10828071818820496No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC18340.10424505887515374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.069.64361
GTATCAA55700.057.989481
ATCAACG70550.044.6852073
TATCAAC72000.044.2819182
CAACGCA72050.043.6721955
ACGCAGA75100.042.115037
CGCAGAG75600.041.8186268
GCAGAGT76700.041.0000089
AACGCAG78250.040.884536
TCAACGC79950.039.729574
GGTTCAC11250.033.8919456
ATTTAGG20700.031.956391
CTGGTTC12400.031.2389764
GTACTGG13950.030.7583751
CAGAGTA80400.030.51666810-11
GTTCACT12500.030.4966727
AGAGTAC80300.030.29510710-11
TTCTCTG8700.030.1242287
GTTCCGA1203.6573525E-529.7971761
ACTGGTT13100.029.1147983
Produced by FastQC (version 0.11.5)