FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482452
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA75470.5090889957988521No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61490.41478577383955767No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA58760.3963703377917126No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC56690.38240698518400595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.26800193193438976No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC39220.2645616856397374No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG38760.26145871839358037No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC36190.24412257530092035No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC33770.22779826935374636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.22199706971962668No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA29640.19993901994803204No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC28400.19157449954534783No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC26670.17990464446740942No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA25990.1753176494078729No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC25730.17356379835569719No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC24370.1643898082366242No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA24320.16405252918812885No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT23710.15993772479648583No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT23030.15535072973694933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.15164066020350067No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC22200.14975189753192683No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT21850.14739094419245952No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA20670.1394311586479697No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA19850.1338997822526463No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA18310.12351158755899011No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA18050.12175773650681437No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA17870.1205435319322312No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA17510.11811512278306481No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC17480.11791275535396761No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA16430.11082989533556567No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA16300.1099529698094778No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG16260.10968314657068155No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA15760.10631035608572824No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG15680.1057707096081357No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT15650.10556834217903852No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC14970.100981347119502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.082.725281
AAGTACG1100.075.711791
GTATCAA46200.058.071471
ATCAACG66700.044.94733
CAACGCA69300.043.2609635
TATCAAC69600.042.989032
AACGCAG73850.041.078886
TCAACGC74250.040.5371254
ACGCAGA77500.038.6836787
CGCAGAG77900.038.4850438
ATTCTCT16200.037.819966
TTCTCTG15750.037.767517
GCAGAGT81600.036.812919
TATTCTC17550.035.5886155
TCTCTGC17100.034.7858628
CTCTGCA17500.033.990769
CTGTGCG2900.030.7674989
ATTTAGG30800.029.9370651
AGAGTAC84300.029.77719510-11
ACTGTGC4850.029.4352958
Produced by FastQC (version 0.11.5)