FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004912463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868694
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA99600.5329925605797418No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC95290.5099283242735301No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC90010.48167329696568834No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA81140.434206991620886No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC64670.3460705712117661No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT62720.3356354758992109No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC57290.30657774895194184No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52360.2801956874694305No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA52350.28014217416013537No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG48740.26082386950458447No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA47160.25236876663595004No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC45980.24605419613912177No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC39440.2110564918600905No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA38330.20511651452832835No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC36600.19585871202026656No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC35380.18933008828625766No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA35160.18815279548176428No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC33690.18028633901537652No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG33410.17878796635511218No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA33260.17798526671568485No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC32220.17241988254898874No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC31870.17054691672365835No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA31730.16979773039352616No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC31710.16969070377493586No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31250.16722909154735874No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG30610.16380423975246886No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC27770.14860645991264487No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG27230.14571674121070652No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT26870.14379026207608095No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT26650.14261296927158754No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT26530.14197080956004568No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT26050.13940217071387825No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA25960.13892055093022185No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG25790.1380108246722042No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT25550.1367265052491205No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC25430.13608434553757867No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA25330.13554921244462712No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT24430.13073301460806316No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA24230.12966274842216008No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG23800.12736167612246843No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA23530.12591681677149924No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT22930.12270601821378994No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA22050.11799684699581633No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC21980.11762225383075026No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA21820.11676604088202776No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG21210.11350172901502334No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT20570.11007687722013342No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA20370.10900661103423032No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA19920.10659851211594834No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA19590.10483257290920824No Hit
TATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGC19520.10445797974414216No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC19400.10381582003260031No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA19090.10215690744445052No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC18960.1014612344236135No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA18930.10130069449572802No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA18810.10065853478418618No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC18700.10006988838193948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)