FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486300
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA152530.6134818807062703No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC132240.5318746732091864No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC123500.49672203676145277No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC104790.42146965370228856No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA102310.41149499255922456No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG98530.396291678397619No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA96260.3871616458190886No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC94200.37887624180509194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86180.3466194747214737No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC85740.34484977677673656No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA74210.2984756465430559No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT72570.29187949965812654No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC69740.2804971242408398No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC62880.25290592446607413No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA61480.24727506736918312No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA58210.23412299400715925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56900.22885412058078267No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG56440.22700398182037568No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA53280.21429433294453606No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA48680.19579294534046573No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC47740.19201222700398182No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC47460.19088605558460364No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA46600.18742710051079917No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT46400.18662269235410048No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT43610.17540119856815348No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG43390.1745163495957849No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG43210.17379238225475607No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC42080.16924747616940836No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC41530.1670353537384869No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA40650.16349595784901258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40550.16309375377066324No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA38310.15408438241563768No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT36990.14877528858142622No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT35730.14370751719422437No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG35120.14125407231629328No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA34570.13904194988537186No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT34350.13815710091300326No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG34090.13711137030929493No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT32830.13204359892209305No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA32570.13099786831838475No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT32560.1309576479105498No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC32080.12902706833447292No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC31410.12633230100953224No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT30570.12295378675139766No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT30330.1219884969633592No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA29990.12062100309697141No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA29960.12050034187346659No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA29950.12046012146563166No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG29800.11985681534810763No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG29380.11816755821904033No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC27790.11177251337328561No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT27020.10867554196999558No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG26390.10614165627639464No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC25930.10429151751598761No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG25500.10256203997908539No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA25220.10143586855970718No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT24930.10026947673249408No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA24880.10006837469331939No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC24870.10002815428548446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.082.614181
GTATCAA89300.064.021781
TCTCTGC23450.049.974028
TTCTCTG23900.048.7841877
ATCAACG120900.047.2380563
CAACGCA124600.046.121795
TATCAAC126450.045.4931452
AACGCAG131650.043.968246
TATTCTC28150.043.5347335
TCAACGC135150.042.6094934
CGCTAGG705.4583423E-542.4940151
ACGCAGA135950.042.2249347
CGCAGAG136400.042.1292428
ATTCTCT29200.041.9692736
GCAGAGT140250.040.972759
CTCTGCA29200.039.929529
GACCGTT3500.039.091357
AGAGTAC144400.031.94675610-11
CAGAGTA144900.030.52282510-11
ACAGCGT1004.4458633E-429.743428
Produced by FastQC (version 0.11.5)